Ikhithi Yokuhlola ye-SARS-COV-2 Isamba Se-Ab (Elisa)

I-SARS-CoV-2 Total Ab Test Kit (ELISA) isuselwe ekuhlolweni kwe-immunoenzymatic ukuze kutholwe amasosha omzimba akwa-SARS-CoV-2 ku-serum yomuntu, i-plasma (EDTA, Heparin noma i-sodium citrate).Imithombo yamapuleti amancane njengesigaba esiqinile ahlanganiswe ne-SARS-CoV-2 recombinant Receptor Binding Domain protein.Esinyathelweni sokuqala sokufukamela esihambisana namasosha omzimba athile (i-SARS-CoV-2-IgG-Ab & nenye i-IgM-Ab) ekhona ezifanekisweni zesiguli abopha kuma-antigen esigabeni esiqinile.Ekupheleni kwe-incubation izingxenye ezingaboshiwe ziyagezwa.Isinyathelo sesibili sokufukamela i-SARS-CoV-2 recombinant Receptor Binding Domain protein conjugate (SARS-CoV-2 recombinant Receptor Binding Domain protein peroxidase conjugate) yengezwa ebophezela ngokukhethekile kumasosha omzimba akwa-SARS-CoV-2 (okubandakanya i-IgG ne-IgM) okuholela ekutheni ukwakheka kwama-immunocomplexes ajwayelekile.Ngemva kwesinyathelo sokugeza sesibili sokususa i-conjugate eyeqile, i-TMB/Substrate iyengezwa (isinyathelo3).Umbala oluhlaza ushintsha ube ophuzi ngemva kokumisa ukusabela ngesixazululo sokumisa.Ukumuncwa kwama-calibrator ne-specimen kunqunywa kusetshenziswa i-ELISA micro plate reader.Imiphumela yamasampuli esiguli itholwa ngokuqhathanisa nevelu yokusika.