I-Measles Virus (MV) IgG ELISA Kit

Incazelo emfushane:

Igciwane lesimungumungwane (MV) IgG ELISA Kit isivivinyo se-immunosorbent esixhumene ne-enzyme ukuze kutholwe ikhwalithi yamasosha omzimba esigaba se-IgG kugciwane leMeasles ku-serum yomuntu noma kuplasma.Kuhloswe ukuthi kusetshenziswe ezindaweni zokucwaninga zomtholampilo ukuze kuxilongwe futhi kuphathwe iziguli ezihlobene nokutheleleka ngegciwane lesimungumungwane.

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Imininingwane Yomkhiqizo

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Isimiso

Le khithi ithola i-Measles virus IgG antibody (MV-IgG) ku-serum yomuntu noma amasampula e-plasma, imicu ye-polystyrene microwell embozwe ngaphambili nge-antigen ye-Measles virus.Ngemva kokwengeza okokuqala amasampula e-serum noma e-plasma azohlolwa, amasosha omzimba ahambisanayo (MV-IgG-Ab & some IgM-Ab) akhona ezibonelweni zesiguli abopha kuma-antigen esigabeni esiqinile, futhi ezinye izingxenye ezingaboshiwe zizosuswa ngokuwashwa.Esinyathelweni sesibili, i-HRP(horseradish peroxidase) -conjugated anti-human IgG izosabela ngokukhethekile kuphela ngama-antibody e-MV IgG.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Ebukhoneni be-(MV Ag) - (MV-IgG) - (anti-human IgG-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi ikhuthaza ukusabela konjiniyela wombala. ukuze kukhiqizwe into eluhlaza okwesibhakabhaka, engeza u-50μl we-Stop Solution, bese uphenduka ubephuzi.Ukuba khona kokumunca kwe-antibody ye-MV-IgG kusampula kunqunywe isifundi se-microplate.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	Indlela Engaqondile
Isitifiketi	I-NMPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T / 96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	Izinyanga ezingu-12