I-Toxoplasma IgG ELISA Kit

Incazelo emfushane:

I-Toxoplasma IgG ELISA Kit iwukuhlolwa kwe-immunosorbent okuxhumene ne-enzyme ukuze kutholwe ikhwalithi yamasosha omzimba e-IgG-class ku-Toxoplasma ku-serum yomuntu noma ku-plasma.Kuhloswe ukuthi kusetshenziswe kuma-laboratories emitholampilo ukuze kuhlolwe futhi kuphathwe iziguli ezihlobene nokutheleleka nge-Toxoplasma.Ngokuvamile, ukwenyuka kwamasosha omzimba e-Toxoplasma IgM kuqala kubonakala ngemva kokutheleleka kwe-Toxoplasma, okungatholakala cishe ezinsukwini eziyisi-6 ngemva kokutheleleka, bese kukhuphuka kancane kancane futhi kuhlala amasonto ambalwa kuya ezinyangeni, futhi ekugcineni kwehle kancane kancane kuze kunyamalale.I-antibody e-positive ye-IgG ibonisa ukutheleleka kwangaphambili kwe-Toxoplasma gondii, ngokuvamile okungamahlalakhona, kodwa futhi kungaba kubi.Ngemuva kokuhlolwa kokuqala kwama-antibodies e-IgG, ukuhlolwa okufanayo kuphindaphindiwe ngemva kwamasonto angu-2-3 futhi uma kukhona ukwanda okuphindwe ka-4 noma ngaphezulu kwamandla we-antibodies, ukuxilongwa okuqhubekayo kuyadingeka.

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Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le kit ithola i-Toxoplasma lgG antibody (TOX-lgG) ku-serum yomuntu noma amasampula e-plasma, imichilo ye-polystyrene microwell embozwe ngaphambili nge-Toxoplasma antigen.Ngemva kokwengeza kuqala amasampula e-serum noma e-plasma okufanele ahlolwe, amasosha omzimba ahambisanayo (TOX-lgG-Ab & some lgM-Ab) akhona kumasampula esiguli abopha ama-antigen esigabeni esiqinile, futhi ezinye izingxenye ezingaboshiwe zizosuswa ngokuwashwa.Esinyathelweni sesibili, i-HRP(horseradish peroxidase) -conjugated anti-human lgG izosabela ngokukhethekile kuphela ngama-antibodies e-TOX lgG.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Ebukhoneni be-(TOX Ag) - (TOX-lgG) - (anti-human lgG-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi ikhuthaza ukusabela konjiniyela wombala. ukuze ukhiqize into eluhlaza okwesibhakabhaka, engeza u-50 µ I we-Stop Solution, bese uphenduka ube phuzi.Ukuba khona kokumunca kwe-antibody ye-TOX-lgG kusampula kunqunywe isifundi se-microplate.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	Indlela engaqondile
Isitifiketi	I-NMPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T / 96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	Izinyanga ezingu-12