I-Mycoplasma Pneumonia IgM ELISA Kit

Incazelo emfushane:

I-Mycoplasma Pneumoniae IgM (MP-IgM) ELISA Kit isivivinyo se-immunosorbent esixhunywe ku-enzyme sokutholwa kwekhwalithi yamasosha omzimba ekilasi le-IgM ku-Mycoplasma Pneumoniae ku-serum yomuntu noma kuplasma.Kuhloswe ukuthi kusetshenziswe kumalabhorethri emitholampilo ukuze kuhlolwe futhi kuphathwe iziguli ezihlobene nokutheleleka nge-Mycoplasma Pneumoniae.I-Mycoplasma pneumoniae (MP) iyi-microorganism ephakathi kwegciwane namagciwane.Incane kakhulu i-prokaryotic cellorganism engakhula futhi yande emithonjeni engaphili.Ngaphezu kwezimpawu, kungase futhi kubangele isifuba somoya, ukuhlasela okunamandla kwezifo ezivimbela amaphaphu, futhi kubangele ukulimala kwesistimu eminingi emithanjeni yenhliziyo, yegazi, nezinzwa ezimaphakathi.Ukuntuleka kokucaciswa kokubonakaliswa komtholampilo futhi akusizi ukuxilongwa.Ngakho-ke, ukusungula indlela yokuxilongwa kusenesikhathi kubaluleke kakhulu ekuqondiseni ukwelashwa komtholampilo.

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Imininingwane Yomkhiqizo

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Isimiso

Le khithi ithola i-Mycoplasma Pneumoniae IgM (MP-IgM) ku-serum yomuntu noma amasampula e-plasma, imichilo ye-polystyrene microwell imbozwa kuqala ngamasosha omzimba aqondiswe kumaprotheni e-immunoglobulin M womuntu (iketango le-anti-μ). Ngemva kokuqala kokwengeza i-serum noma izibonelo ze-plasma okufanele zihlolwe. , amasosha omzimba e-IgM kusifanekiso angabanjwa, nezinye izingxenye ezingaboshiwe (kuhlanganise namasosha omzimba athile e-IgG) azokhishwa ngokuwashwa.Esinyathelweni sesibili, i-HRP(horseradish peroxidase)-conjugated antigens izosabela ngokukhethekile ngama-antibody e-MP IgM.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Ebukhoneni be-(anti-μ) - (MP-IgM) - (MP-Ag-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi ikhuthaza ukusabela konjiniyela wombala. ukuze ukhiqize into eluhlaza okwesibhakabhaka, engeza u-50μl we-Stop Solution, bese uphenduka ube phuzi.Ukuba khona kokumunca kwe-antibody ye-MP-IgM kusampula kunqunywe isifundi se-microplate.

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Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	Indlela Yokuthwebula
Isitifiketi	I-NMPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	Izinyanga ezingu-12