I-Anti-SARS-CoV-2 Neutralizing Antibody Test Kit (ELISA)

Incazelo emfushane:

Lo mkhiqizo usebenzisa i-enzyme-linked immunosorbent assay ukuze kutholwe ngokufanelekile noma yimaphi amasosha omzimba ku-serum ne-plasma angenza kube lula ukusebenzisana kwe-RBD-ACE2, futhi le ndlela ayinqunyelwe isampula yezinhlobo zohlobo kanye nezigaba ezingaphansi zamasosha omzimba.Amasosha omzimba angathathi hlangothi angavimba i-viral spike glycoprotein receptor binding region (RBD) ukuthi ihlangane ne-cell surface receptor angiotensin-converting enzyme-2 (ACE2).

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Ikhithi isebenzisa umgomo wokuhlolwa komncintiswano ukuthola amasosha omzimba angathathi hlangothi ngokumelene ne-SARS-CoV-2 kumasampula e-serum noma e-plasma.Okokuqala, isampula elizohlolwa, ukulawula okuphozithivu, kanye nokulawula okunegethivu kuhlanganiswa ne-HRP-RBD ukuze i-antibody engathathi hlangothi ibophezele ku-HRP-RBD, bese ingxube yengezwa epuleti lokuthwebula elimbozwe ngaphambili ngamaprotheni e-hACE2.I-HRP-RBD engaboshelwe ekwenzeni amasosha omzimba angasebenzi kanye nanoma iyiphi i-HRP-RBD eboshelwe kumasosha omzimba angathathi hlangothi izothwetshulwa epuleti elimboziwe, kuyilapho i-HRP-RBD eyinkimbinkimbi eboshelwe ekwenzeni amasosha omzimba angasebenzi ikhishwa ngesikhathi iwashwa.Isixazululo se-TMB sabe seyengezwa ukuze kuthuthukiswe umbala.Ekugcineni, isixazululo sokumisa sengeziwe futhi ukusabela kwanqanyulwa.Ukuba khona noma ukungabikho kwe-Anti-SARS-CoV-2 antibody ehlukanisayo kusampula kwahlulelwa ngokutholwa kwe-absorbence (A-value) ngumfundi we-microplate.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	Indlela yokuncintisana
Isitifiketi	CE
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	Izinyanga ezingu-12