I-TB-IGRA Diagnostic Test

Isithombe esifakiwe sokuhlolwa kokuxilonga kwe-TB-IGRA

Incazelo emfushane:

Ukuhlolwa kokuxilonga kwe-TB-IGRA, okubuye kubizwe nge-Interferon Gamma Release Assay, i-ELISA yokutholwa komthamo we-Interferon Gamma (IFN-γ) esabela ekuvuselelweni kwe-in vitro ngama-antigen e-Mycobacterium Tuberculosis kumasampuli egazi lomuntu.I-TB-IGRA ikala ukusabela komzimba womuntu ku-Mycobacterium Tuberculosis.Ukuhlolwa kuhloswe ukuthi kusetshenziswe njengosizo ekuhlonzeni ukutheleleka nge-TB okuhlanganisa kokubili ukutheleleka kweSifo Sofuba esicashile kanye nesifo Sofuba.

Ukusabela komzimba okubangelwa isifo sofuba iMycobacterium ngokuvamile kuyimpendulo yeselula.Ngemva kokutheleleka nge-Mycobacterium tuberculosis, umzimba ukhiqiza amangqamuzana athile enkumbulo T ajikeleza egazini le-peripheral.Ukukalwa kwe-IFN-γ ngokumelene nesifo sofuba se-Mycobacterium kuboniswe njengendlela ephumelelayo yokuthola ukutheleleka kwe-TB (kokubili okucashile nokusebenzayo), okubizwa nge-IFN-γ in vitro release assay (IGRA).Umehluko omkhulu ekuhlolweni kwe-tuberculin (TST) ukuthi i-IGRA ikhetha ama-antigen athile akhona kuphela ku-Mycobacterium tuberculosis kodwa awekho ku-BCG kanye nama-mycobacteria amaningi angewona ama-tuberculous.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Ikhithi isebenzisa i-interferon-γ yokukhululwa kwe-assay ye-Mycobacterium tuberculosis (TB-IGRA) ukukala ukushuba kokusabela kokuzivikela komzimba kweselula okulamulwa yi-Mycobacterium tuberculosis specific antigen.
I-Enzyme-linked immunosorbent assay kanye nomgomo wesamishi we-antibody kabili.
• Ama-microplates ambozwe ngaphambili ngamasosha omzimba e-IFN-γ.
• Izibonelo ezizohlolwa zengezwa emithonjeni ye-microplate embozwe ngamasosha omzimba, bese kungezwa amasosha omzimba e-horseradish peroxidase (HRP) -conjugated anti IFN-γ emithonjeni efanele.
• I-IFN-γ, uma ikhona, izokwakha isangweji eliyinkimbinkimbi enamasosha omzimba e-IFN-γ kanye namasosha omzimba e-HRP-conjugated anti IFN-γ.
• Umbala uzothuthukiswa ngemva kokwengeza izixazululo ze-substrate, futhi uzoshintsha ngemva kokwengeza izixazululo zokumisa.I-absorbence (OD) ikalwa ngomfundi we-ELISA.
• Ukugxiliswa kwe-IFN-γ kusampula kuhlotshaniswa ne-OD enqunyiwe.

Izici Zomkhiqizo

I-ELISA yokuxilonga esebenzayo yokutheleleka nge-TB equbisile futhi esebenzayo

Akukho ukuphazamiseka komgomo we-BCG

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	Indlela ye-sandwich
Isitifiketi	CE, NMPA
Isifanekiso	Igazi lonke
Ukucaciswa	48T (thola amasampula ayi-11);96T (bona amasampula angama-27)
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	Izinyanga ezingu-12