I-Hepatitis E virus IgM ELISA Kit

Incazelo emfushane:

Igciwane le-Hepatitis E I-IgM Test Cassette isetshenziselwa ukuhlonza ngokwekhwalithi amasosha omzimba we-Hepatitis E Virus IgM ku-serum yomuntu, i-plasma (EDTA, i-heparin, i-sodium citrate) noma igazi eliphelele (EDTA, i-heparin, i-sodium citrate).Ukuhlolwa kuzosetshenziswa njengosizo ekuxilongweni kwesifo sokusha kwesibindi kohlobo lwe-viral, esibangelwa i-Hepatitis E Virus.

Igciwane le-Hepatitis E (HEV) igciwane le-RNA elingamboziwe, elinemicu eyodwa elisakazwa ikakhulukazi ngomzila we-fecal-oral, ukumpontshelwa igazi futhi mhlawumbe nengane yomama.Ukutheleleka nge-HEV kubangela i-acute sporadic kanye ne-epidemic viral Hepatitis futhi kudala izifo zesibindi ezinzima noma ezincane ezifana ne-Hepatitis A. Nakuba kunezinhlobo ezine ezinkulu ze-HEV, kune-serotype eyodwa kuphela.

Ukutheleleka nge-HEV kubantu kukhiqiza amasosha omzimba e-IgM, IgA kanye ne-IgG.I-HEV-IgM kanye ne-HEV-IgA positivity iwuphawu lokutheleleka nge-HEV okuyingozi noma kwakamuva.Ukuthi i-anti-HEV-IgM kanye ne-anti-HEV-IgA ilungile kokukodwa noma kokubili, kuyinkomba yokutheleleka kwe-HEV kwakamuva.Ukuba khona kokutheleleka kwe-HEV kwakamuva, kuhlanganiswe nokusebenza kwesibindi, kungasetshenziswa ukuze kutholwe ukuthi ukutheleleka kushubile noma kwakamuva.Ukuba khona kokutheleleka nge-HEV esibindini kungasetshenziswa ukuze kutholwe ukuthi lesi sifo siyisifo sokusha kwesibindi kohlobo E noma ukululama ku-acute hepatitis E.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le khithi ithola igciwane le-hepatitis E i-IgM antibody (HEV-IgM) ku-serum yomuntu noma amasampula e-plasma, imichilo ye-polystyrene microwell embozwe ngaphambili ngamasosha omzimba aqondiswe kumaprotheni womuntu we-immunoglobulin M (anti-μ chain).Ngemva kokwengeza kuqala i-serum noma izibonelo ze-plasma ezizohlolwa, amasosha omzimba e-IgM kusampula angabanjwa, futhi ezinye izingxenye ezingaboshiwe (kuhlanganise namasosha omzimba athile e-IgG) zizosuswa ngokuwashwa.Esinyathelweni sesibili, i-HRP (horseradish peroxidase) -conjugated antigens izosabela ngokukhethekile ngama-antibody e-HEV IgM.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Ebukhoneni be-(anti-μ) - (HEV-IgM) - (HEV Ag-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi igqugquzela ukusabela konjiniyela wombala khiqiza into eluhlaza okwesibhakabhaka, engeza u-50μl we-Stop Solution, bese uphenduka ube phuzi.Ukuba khona kokumunca kwe-antibody ye-HEV-IgM kusampula kunqunywe yisifundi se-microplate.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo Indlela yokuthwebula
Isitifiketi CE
Isifanekiso I-serum yomuntu / i-plasma
Ukucaciswa 48T / 96T
Izinga lokushisa lesitoreji 2-8℃
Impilo yeshelufu Izinyanga ezingu-12

Uku-oda Ulwazi

Igama lomkhiqizo Pakisha Isifanekiso
I-Hepatitis E virus IgM ELISA Kit 48T / 96T I-serum yomuntu / i-plasma

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