I-Hepatitis E virus IgM ELISA Kit
Isimiso
Le khithi ithola igciwane le-hepatitis E i-IgM antibody (HEV-IgM) ku-serum yomuntu noma amasampula e-plasma, imichilo ye-polystyrene microwell embozwe ngaphambili ngamasosha omzimba aqondiswe kumaprotheni womuntu we-immunoglobulin M (anti-μ chain).Ngemva kokwengeza kuqala i-serum noma izibonelo ze-plasma ezizohlolwa, amasosha omzimba e-IgM kusampula angabanjwa, futhi ezinye izingxenye ezingaboshiwe (kuhlanganise namasosha omzimba athile e-IgG) zizosuswa ngokuwashwa.Esinyathelweni sesibili, i-HRP (horseradish peroxidase) -conjugated antigens izosabela ngokukhethekile ngama-antibody e-HEV IgM.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Ebukhoneni be-(anti-μ) - (HEV-IgM) - (HEV Ag-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi igqugquzela ukusabela konjiniyela wombala khiqiza into eluhlaza okwesibhakabhaka, engeza u-50μl we-Stop Solution, bese uphenduka ube phuzi.Ukuba khona kokumunca kwe-antibody ye-HEV-IgM kusampula kunqunywe yisifundi se-microplate.
Izici Zomkhiqizo
Ukuzwela okuphezulu, ukucaciswa nokuzinza
Ukucaciswa Komkhiqizo
Isimiso | Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent |
Uhlobo | Indlela yokuthwebula |
Isitifiketi | CE |
Isifanekiso | I-serum yomuntu / i-plasma |
Ukucaciswa | 48T / 96T |
Izinga lokushisa lesitoreji | 2-8℃ |
Impilo yeshelufu | Izinyanga ezingu-12 |
Uku-oda Ulwazi
Igama lomkhiqizo | Pakisha | Isifanekiso |
I-Hepatitis E virus IgM ELISA Kit | 48T / 96T | I-serum yomuntu / i-plasma |