I-Hepatitis D virus IgM ELISA Kit

Igciwane le-Hepatitis D IgM ELISA Kit Isithombe Esifakiwe

Incazelo emfushane:

Igciwane le-Hepatitis D I-IgM ELISA Kit isivivinyo se-immunosorbent esixhumene ne-enzyme sokutholwa kwekhwalithi yamasosha omzimba esigaba se-IgM kugciwane le-hepatitis D ku-serum yomuntu noma kuplasma.Kuhloswe ukuthi kusetshenziswe kuma-laboratories emitholampilo ukuze kuhlolwe futhi kuphathwe iziguli ezihlobene nokutheleleka ngegciwane le-hepatitis D.I-anti-HDV-IgM enhle ibonisa ukutheleleka kwe-HDV okunamandla noma kwakamuva;ukutheleleka okuhlangene ne-HDV/HBV kuhlotshaniswa ne-anti-HDV-IgM enhle yesikhashana;ukutheleleka okweqayo kuhlotshaniswa ne-anti-HDV-IgM eqhubekayo enhle;ngakho-ke, ukuhlolwa kwe-anti-HDV-IgM kunenani elibalulekile lokuxilonga.Amasosha omzimba e-Anti-HDV-IgM angakwazi ukuhlukanisa phakathi kwezifo zakamuva noma zangaphambilini, izifo ezihlangene kanye nezifo ezeqayo, kanye nezihloko eziqhubekayo ze-anti-HDV-IgG ne-IgM zishintshashintsha ngokuphindaphindiwe, okuyizinkomba ezibalulekile zezifo ze-HDV ezingapheli, ngakho ukutholakala kwe-anti-HDV-IgG kanye ne-IgM I-HDV-IgM ibalulekile ekuxilongweni nasekubikezelweni kwezifo ze-HDV.

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Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le khithi ithola igciwane le-hepatitis D i-IgM antibody (HDV-IgM) ku-serum yomuntu noma amasampula e-plasma, imichilo ye-polystyrene microwell embozwe ngaphambili ngamasosha omzimba aqondiswe kumaprotheni womuntu we-immunoglobulin M (anti-μ chain).Ngemva kokwengeza kuqala i-serum noma izibonelo ze-plasma ezizohlolwa, amasosha omzimba e-IgM kusampula angabanjwa, futhi ezinye izingxenye ezingaboshiwe (kuhlanganise namasosha omzimba athile e-IgG) zizosuswa ngokuwashwa.Esinyathelweni sesibili, i-HRP (horseradish peroxidase) -conjugated antigens izosabela ngokukhethekile ngama-antigen e-HDV IgM.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Lapho kukhona (anti-μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi ikhuthaza ukusabela konjiniyela wombala khiqiza into eluhlaza okwesibhakabhaka, engeza u-50μl we-Stop Solution, bese uphenduka ube phuzi.Ukuba khona kokumunca kwe-antibody ye-HDV-IgM kusampula kunqunywe isifundi se-microplate.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	Indlela yokuthwebula
Isitifiketi	I-NMPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T / 96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	Izinyanga ezingu-12