I-Rubella virus IgM ELISA Kit

Incazelo emfushane:

I-Rubella virus IgM (RV-IgM) ELISA Kit isivivinyo se-immunosorbent esixhumene ne-enzyme sokutholwa kwekhwalithi yamasosha omzimba e-IgM-class ku-Rubella virus ku-serum yomuntu noma ku-plasma.I-anti-rubella virus Amasosha omzimba e-IgM ku-serum yomuntu noma ku-plasma ahlobene eduze nokutheleleka okunamandla ngegciwane, futhi amasosha omzimba aqondene negciwane le-rubella e-IgM angenye yezinkomba ezibucayi neziqondile zokuxilongwa elabhorethri.Ukutholwa kwegciwane le-rubella IgM kubalulekile kuma-eugenics kanye nesiqondiso somtholampilo.Ukuhlolwa kwe-antibody ye-Rubella virus IgM akufanele kusetshenziselwe ukuhlolwa kwangaphambi kokubeletha kwabantu abangenazimpawu, futhi imiphumela yokuhlolwa akufanele isetshenziswe iyodwa njengesisekelo sokunqanyulwa kokukhulelwa.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le khithi ithola i-Rubella virus IgM antibody (RV-IgM) ku-serum yomuntu noma amasampula e-plasma, imichilo ye-polystyrene microwell embozwe ngaphambili ngamasosha omzimba aqondiswe kuphrotheni yomuntu ye-immunoglobulin M (anti-µ chain).Ngemva kokwengeza kuqala i-serum noma izibonelo ze-plasma ezizohlolwa, amasosha omzimba e-IgM kusampula angabanjwa, futhi ezinye izingxenye ezingaboshiwe (kuhlanganise namasosha omzimba athile e-IgG) zizosuswa ngokuwashwa.Esinyathelweni sesibili, i-HRP (horseradish peroxidase)-conjugated antigens izosabela ngokukhethekile kuphela ngama-antibodies e-RV IgM.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Lapho kukhona (anti-µ) -(RV-lgM) -(RV Ag-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi igqugquzela ukusabela konjiniyela wombala khiqiza into eluhlaza okwesibhakabhaka, engeza u-50 µI we-Stop Solution, bese uphenduka ube phuzi.Ukuba khona kokumunca kwe-antibody ye-RV-IgM kusampula kunqunywe isifundi se-microplate.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	Indlela yokuthwebula
Isitifiketi	I-NMPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T / 96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	Izinyanga ezingu-12