I-Human Cytomegalovirus IgM ELISA Kit

Incazelo emfushane:

I-Human Cytomegalovirus IgM (HCMV-IgM) ELISA Kit isivivinyo se-immunosorbent esixhumene ne-enzyme sokutholwa kwekhwalithi yamasosha omzimba e-IgM-class ku-Human Cytomegalovirus ku-serum yomuntu noma kuplasma.Kuhloswe ukuthi kusetshenziswe kuma-laboratories emitholampilo ukuze kuhlolwe futhi kuphathwe iziguli ezihlobene nokutheleleka nge-Human Cytomegalovirus.I-HCMV ingenye yezimbangela ezibaluleke kakhulu zokukhubazeka kokuzalwa kubantu.Ukutheleleka okuyisisekelo noma okusha okuphindaphindayo kwabesifazane abakhulelwe kungabangela izifo ze-intrauterine noma ze-perinatal ezinganeni ezisanda kuzalwa.Amaphesenti angu-80 wezingane ezitheleleke nge-HCMV ngesikhathi sokukhulelwa kokuqala zingaba nezimpawu ezifana nokukhubazeka kwengqondo, ukungaboni kahle, nokungezwa.Ukutheleleka okuyisisekelo kwe-HCMV kunemiphumela emibi kakhulu futhi esongela ukuphila ezigulini ezicindezelekile, futhi izinga lokufa kwenyumoniya ye-interstitial ngenxa yokutheleleka kwe-HCMV ekufakweni komnkantsha liphezulu njengo-75%.Muva nje, i-HCMV itholakale ingomunye wemithombo yezifo ezingosomathuba ezigulini ezinengculazi.

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Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le khithi ithola i-Human Cytomegalovirus IgM antibody (HCMV-IgM) ku-serum yomuntu noma amasampula e-plasma, imichilo ye-polystyrene microwell embozwe ngaphambili ngamasosha omzimba aqondiswe kuphrotheni yomuntu ye-immunoglobulin M (anti-µ chain).Ngemva kokwengeza kuqala i-serum noma izibonelo ze-plasma ezizohlolwa, amasosha omzimba e-IgM kusampula angabanjwa, futhi ezinye izingxenye ezingaboshiwe (kuhlanganise namasosha omzimba athile e-IgG) zizosuswa ngokuwashwa.Esinyathelweni sesibili, i-HRP (horseradish peroxidase) -conjugated antigens izosabela ngokukhethekile kuphela ngama-antibody e-HCMV IgM.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Ebukhoneni be-(anti-µ) -(HCMV-lgM) -(HCMV Ag-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi ivuselela ukusabela konjiniyela wombala khiqiza into eluhlaza okwesibhakabhaka, engeza u-50 µI we-Stop Solution, bese uphenduka ube phuzi.Ukuba khona kokumunca kwe-antibody ye-HCMV-IgM kusampula kunqunywe isifundi se-microplate.

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Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	Indlela yokuthwebula
Isitifiketi	I-NMPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T / 96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	Izinyanga ezingu-12