I-Herpes Simplex virus I-IgM ELISA Kit
Isimiso
Le kit ithola i-Herpes Simplex Virus I lgM antibody (HSV1-lgM) ku-serum yomuntu noma amasampula e-plasma, imichilo ye-polystyrene microwell embozwe ngaphambili ngamasosha omzimba aqondiswe kumaprotheni womuntu we-immunoglobulin M (anti-µ chain).Ngemva kokwengeza kuqala i-serum noma izibonelo ze-plasma ezizohlolwa, amasosha omzimba e-lgM kusampula angabanjwa, nezinye izingxenye ezingaboshiwe (okuhlanganisa amasosha omzimba athile e-lgG) azosuswa ngokuwashwa.Esinyathelweni sesibili, i-HRP (i-horseradish peroxidase) -ama-antigen ahlanganisiwe azosabela ngokukhethekile ngama-antibodies e-HSV1 lgM.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Ebukhoneni be-(anti-µ) - (HSV1-lgM) - (HSV1 Ag-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi ivuselela ukusabela konjiniyela wombala khiqiza into eluhlaza okwesibhakabhaka, engeza u-50 µ I we-Stop Solution, bese uphenduka ube phuzi.Ukuba khona kokumunca kwe-antibody ye-HSV1-lgM kusampula kunqunywe isifundi se-microplate.
Izici Zomkhiqizo
Ukuzwela okuphezulu, ukucaciswa nokuzinza
Ukucaciswa Komkhiqizo
Isimiso | Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent |
Uhlobo | Indlela yokuthwebula |
Isitifiketi | I-NMPA |
Isifanekiso | I-serum yomuntu / i-plasma |
Ukucaciswa | 48T / 96T |
Izinga lokushisa lesitoreji | 2-8℃ |
Impilo yeshelufu | Izinyanga ezingu-12 |
Uku-oda Ulwazi
Igama lomkhiqizo | Pakisha | Isifanekiso |
I-Herpes Simplex virus I-IgM ELISA Kit | 48T / 96T | I-serum yomuntu / i-plasma |