I-Herpes Simplex II IgM ELISA Kit

Incazelo emfushane:

I-Herpes Simplex II IgM ELISA Kit iyi-enzyme-linked immunosorbent assay yokutholwa kwekhwalithi yamasosha omzimba e-IgM-class ku-Herpes Simplex virus II ku-serum yomuntu noma i-plasma.I-Herpes Simplex Virus (HSV) ingeyomndeni we-A virus womndeni wakwa-Herpesviridae futhi inosayizi wezinhlayiyana ezingaba ngu-180 nm.Njengamanje leli gciwane lihlukaniswa njengohlobo I kanye nohlobo II ngokusekelwe ekwehlukeni kwe-antigenicity.Amagciwane e-Herpes simplex asabalele kubantu futhi abantu yibona kuphela abasingathi.Uhlobo lwe-HSV II lusakazwa ikakhulukazi ngocansi.Ihlotshaniswa kakhulu nezifo zangaphandle ezithweni zangasese kanye nezifo ezisanda kuzalwa futhi zingabangela i-herpes ezithweni zangasese kanye ne-neonatal herpes.Uma owesifazane okhulelwe enokutheleleka kwe-herpes virus eyinhloko, igciwane lingase lithelele ingane nge-placenta futhi lenze ukutheleleka kokuzalwa.Uma ukutheleleka okuyinhloko noma igciwane le-herpes eliphindaphindiwe likhona emgodini wokuzalwa komama, lingangena usana olusanda kuzalwa ngesikhathi sokubeletha futhi libangele ukutheleleka kwengane.

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Isimiso

Le kit ithola i-Herpes Simplex virus II IgM antibody (HSV2-IgM) ku-serum yomuntu noma amasampula e-plasma, imichilo ye-polystyrene microwell embozwe ngaphambili ngamasosha omzimba aqondiswe kuphrotheni yomuntu ye-immunoglobulin M (anti-µ chain).Ngemva kokwengeza kuqala i-serum noma izibonelo ze-plasma ezizohlolwa, amasosha omzimba e-IgM kusampula angabanjwa, futhi ezinye izingxenye ezingaboshiwe (kuhlanganise namasosha omzimba athile e-IgG) zizosuswa ngokuwashwa.Esinyathelweni sesibili, i-HRP (horseradish peroxidase)-conjugated antigens izosabela ngokukhethekile kuphela ngama-antibodies e-HSV2 IgM.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Ebukhoneni be-(anti-µ)-(HSV2-lgM)-(HSV2 Ag-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi ivuselela ukusabela konjiniyela wombala khiqiza into eluhlaza okwesibhakabhaka, engeza u-50 µ I we-Stop Solution, bese uphenduka ube phuzi.Ukuba khona kokumunca kwe-antibody ye-HSV2-IgM kusampula kunqunywe isifundi se-microplate.

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Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	Indlela yokuthwebula
Isitifiketi	I-NMPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T / 96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	Izinyanga ezingu-12