Ikhithi Yokuhlola Esheshayo ye-H. Pylori IgG (igolide eli-Colloidal)

Ikhithi Yokuhlola Esheshayo ye-H. Pylori IgG (igolide le-Colloidal) iyi-lateral flow chromatographic immunoassay.Amakhasethi okuhlola aqukethe :1) iphedi ye-conjugate enombala oyi-burgundy equkethe i-antibody ye-IgG yegundane ehlanganiswe negolide eliyi-colloidal;2) i-nitrocellulose membrane strip equkethe umugqa owodwa wokuhlola (umugqa we-T) kanye nomugqa wokulawula (umugqa C).Umugqa we-T uhlanganiswe ngaphambili ne-antigen ye-H. Pylori.Ulayini u-C uhlanganiswe ne-anti-mouse IgG antibody.Lapho ivolumu eyanele yesampula yokuhlola yengezwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sithuthwa isenzo se-capillary kulwelwesi olumbozwe ngaphambili.

I-antibody ye-H. Pylori IgG uma ikhona kusifanekiso izobophezela kuma-conjugates e-IgG egundane.I-immunocomplex ibe isithathwa i-antibody eboshwe emugqeni we-T, yenza umugqa we-T onombala o-burgundy, okubonisa imiphumela yokuhlolwa kwe-H. Pylori IgG.Ukuze usebenze njengesilawuli senqubo, ulayini onombala uzohlala uvela endaweni yomugqa wokulawula obonisa ukuthi umthamo ofanele wesifanekiso sengeziwe futhi i-membrane wicking yenzekile.