I-Enterovirus 71(EV71) IgM ELISA Kit

Incazelo emfushane:

I-Enterovirus 71 IgM (EV71-IgM) Ikhithi ye-ELISA iyi-assay ye-enzyme-linked immunosorbent yokuthola ikhwalithi yamasosha omzimba e-IgM-class ku-Enterovirus 71 ku-serum yomuntu noma i-plasma.Kuhloswe ukuthi kusetshenziswe kumalabhorethri emitholampilo ukuze kuhlolwe futhi kuphathwe iziguli ezihlobene nokutheleleka nge-Enterovirus 71.

I-human enterovirus 71 (EV71), ilungu elisha kakhulu le-Enteroviridae, iyimbangela evamile yesifo sesandla, sonyawo kanye nomlomo (HFMD) futhi ngezinye izikhathi ihlotshaniswa nezifo ezinzima zesistimu yezinzwa.Ukutheleleka kuvame ukukhuphuka ngoJuni noJulayi.Ukutheleleka nge-EV71 kungase kungabi nazimpawu noma kubangele isifo sohudo nokuqubuka.Izingane ezingaphansi kweminyaka emihlanu zisengozini enkulu yokuba nezifo ezimbi kakhulu kunabantu abadala kanye nezingane ezindala.

I-EV71 idluliselwa isuka kumuntu iye komunye ngokuthintana ngqo nangokungcoliswa kwezandla noma izinto ezinendle ethelelekile.Ukuphuma kwekhala nomphimbo, amathe noma uketshezi oluphuma kumabhamuza nakho kungasabalalisa igciwane.

I-EV71 ingahlukaniswa futhi ibonwe emphinjeni nasezindleleni kusetshenziswa ukuhlolwa kwe-PCR.I-Viral RNA itholwe oketshezini lwe-vesicle yesikhumba, igazi nomchamo.Ukuxilongwa kwangaphambi kwesikhathi futhi kulula kungafinyelelwa kusetshenziswa ama-EV71-specific serological assays, okuhlanganisa nokuhlola okuqondene ne-IgM antibody.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le kit ithola i-Enterovirus 71 IgM antibody (EV71-IgM) ku-serum yomuntu noma amasampula e-plasma, imichilo ye-polystyrene microwell imbozwe ngaphambili ngamasosha omzimba aqondiswe kumaprotheni e-immunoglobulin M womuntu (iketango le-anti-μ). Uma kuhlolisiswa, amasosha omzimba e-IgM kusampula angabanjwa, nezinye izingxenye ezingaboshiwe (kuhlanganise namasosha omzimba athile e-IgG) azokhishwa ngokuwashwa.Esinyathelweni sesibili, i-HRP (horseradish peroxidase) -conjugated antigens izosabela ngokukhethekile ngama-antibody EV71 IgM.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Ebukhoneni be-(anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi ikhuthaza ukusabela konjiniyela wombala. ukuze ukhiqize into eluhlaza okwesibhakabhaka, engeza u-50μL we-Stop Solution, bese uphenduka ube phuzi.Ukuba khona kokumunca kwe-antibody EV71-IgM kusampula kunqunywe isifundi se-microplate.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	Indlela yokuthwebula
Isitifiketi	CE
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T / 96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	Izinyanga ezingu-12