I-Enterovirus 71(EV71) IgG ELISA Kit

Incazelo emfushane:

I-Human Enterovirus 71 IgG ELISA Kit iyi-enzyme-linked immunosorbent assay yokutholwa kwekhwalithi yamasosha omzimba e-IgG-class ku-Enterovirus 71 ku-serum yomuntu noma i-plasma.Kuhloswe ukuthi kusetshenziswe kumalabhorethri emitholampilo ukuze kuhlolwe futhi kuphathwe iziguli ezihlobene nokutheleleka nge-Enterovirus 71.

Ukubonakaliswa okuyinhloko komtholampilo kwe-EV71 isifo sesandla, unyawo nomlomo.Ezinye izingane ezinokutheleleka kwe-EV71 ziba ne-herpetic pharyngitis, futhi ezimweni ezimbi kakhulu, i-encephalitis yegciwane, i-viral encephalomyelitis, i-pulmonary edema kanye nokopha kwamaphaphu.

Ukuxilongwa okuqondile kokutheleleka kwe-EV71 kuncike ekutholweni kwamasosha omzimba e-EV71-RNA, EV71-IgM kanye ne-EV71-IgG ku-serum, noma i-EV71-RNA kuma-swab specimens.

I-EV71-IgM iyinkomba ebalulekile yokutheleleka okuyinhloko noma kwakamuva futhi isiza ukutholwa kusenesikhathi nokwelashwa kwe-EV71.I-EV71-IgG iyinkomba ebalulekile yokuxilongwa okuhlukile kokutheleleka futhi ingasetshenziselwa uphenyo lwe-epidemiological kanye nokuhlolwa kokugoma.Ingasetshenziselwa uphenyo lwe-epidemiological kanye nokuhlola umphumela wokugoma.Ukushintsha kwe-antibody titers kukho kokubili okutholakele kanye negazi elibukhali kunganquma futhi ukutheleleka kwe-EV71, futhi uma isihloko se-serum antibody sikhuphuka ngokwejometri ngo-4 esikhathini sokululama uma kuqhathaniswa nesikhathi esibucayi, kungabhekwa njengokwethula ukutheleleka nge-EV71.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le kit ithola i-Enterovirus 71 IgG antibody (EV71-IgG) ku-serum yomuntu noma amasampula e-plasma, imicu ye-polystyrene microwell embozwe ngaphambili nge-Enterovirus 71 antigen.Ngemva kokwengeza kuqala amasampula e-serum noma e-plasma azohlolwa, amasosha omzimba athile ahambisanayo (EV71-IgG-Ab & some IgM-Ab) akhona kumasampula esiguli abopha kuma-antigen esigabeni esiqinile, futhi ezinye izingxenye ezingaboshiwe zizosuswa ngokuwashwa.Esinyathelweni sesibili, i-HRP (horseradish peroxidase) -conjugated anti-human IgG izosabela kuphela ngama-antibody EV71 IgG.Ngemva kokugeza ukuze kukhishwe i-HRP-conjugate engaboshiwe, izixazululo ze-chromogen zengezwa emithonjeni.Ebukhoneni be-(EV71 Ag) - (EV71-IgG) - (anti-human IgG-HRP) immunocomplex, ngemva kokugeza ipuleti, i-substrate ye-TMB yengezwa ukuze kuthuthukiswe umbala, futhi i-HRP exhunywe kwinkimbinkimbi ivuselela ukusabela konjiniyela wombala. ukuze ukhiqize into eluhlaza okwesibhakabhaka, engeza u-50μL we-Stop Solution, bese uphenduka ube phuzi.Ukuba khona kokumunca kwe-antibody ye-EV71-IgG kusampula kunqunywe isifundi se-microplate.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	Indlela engaqondile
Isitifiketi	CE
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T / 96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	Izinyanga ezingu-12