Ikhithi Yokuhlola Esheshayo ye-Chlamydia Trachomatis Antigen Rapid(Colloidal Gold)

Ikhithi Yokuhlola Esheshayo ye-Chlamydia Trachomatis Antigen Rapid (igolide le-Colloidal) i-lateral flow chromatographic immunoassay.Amakhasethi okuhlola ahlanganisa :1) iphedi ye-conjugate enombala oyi-burgundy equkethe i-anti-Chlamydia Trachomatis Lipopolysaccharide monoclonal antibody II ehlanganiswe negolide eliyi-colloidal;

I-nitrocellulose membrane strip equkethe umugqa owodwa wokuhlola (umugqa we-T) kanye nolayini wokulawula (umugqa C).Ulayini we-T uhlanganiswe ngaphambili ne-anti-Chlamydia Trachomatis Lipopolysaccharide monoclonal antibody I. Ulayini u-C uboshwe ngaphambili ne-anti-mouse polyclonal antibody.Lapho ivolumu eyanele yesampula yokuhlola yengezwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sithuthwa isenzo se-capillary kulwelwesi olumbozwe ngaphambili.

I-Chlamydia Trachomatis Antigen uma ikhona kusifanekiso izobophezela kuma-conjugates e-anti-Chlamydia Trachomatis Lipopolysaccharide monoclonal antibody II.I-immunocomplex ibe isithathwa i-antibody embozwe emugqeni we-T, yenza umugqa we-T burgundy, okubonisa imiphumela yokuhlolwa kwe-Chlamydia Trachomatis antigen positive.Ukuze usebenze njengesilawuli senqubo, ulayini onombala uzohlala uvela endaweni yomugqa wokulawula obonisa ukuthi umthamo ofanele wesifanekiso sengeziwe futhi i-membrane wicking yenzekile.