I-Anti-Zona Pellucida (ZP) Antibody ELISA Kit

Incazelo emfushane:

Lo mkhiqizo uklanyelwe ukutholwa kwekhwalithi ye-in vitro yamazinga e-zona pellucida (ZP) amasosha omzimba ku-serum yomuntu. I-zona pellucida, i-matrix ekhethekile ye-extracellular ezungeze i-oocyte, idlala indima ebalulekile ekuqapheleni kwesidoda, ukubopha, nokuvundiswa, kanye nokukhula kombungu kusenesikhathi.

Amasosha omzimba e-ZP ama-autoantibodies aqondise ama-antigen e-zona pellucida. Lapho zivela emzimbeni, zingabopha ngokuqondile ku-zona pellucida, zivimbele ukusebenzisana okuvamile phakathi kwesidoda nama-oocyte, ngaleyo ndlela kuvimbele ukuvundiswa. Ngaphezu kwalokho, zingase ziphazamise inqubo yokufakelwa kwamaqanda avundisiwe, okungenye yezimbangela ezibalulekile zokungazali kwe-autoimmune.

Ngokomtholampilo, lokhu kutholwa kusebenza njengendlela yokuhlola eyisizayo yokungazali kwe-autoimmune. Ngokuthola izinga lamasosha omzimba e-ZP ku-serum yeziguli, inganikeza ulwazi olubalulekile lwereferensi ukuze kucaciswe izimbangela zokungazali kwezinye iziguli ezinezimbangela ezingaziwa, kusize odokotela bakhe izinhlelo zokuxilonga nezokwelashwa ezihlosiwe.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le khithi ithola amasosha omzimba e-zona pellucida (ZP-Ab) kumasampuli e-serum yomuntu ngokusekelwe endleleni engaqondile, nge-zona pellucida ehlanziwe esetshenziswa njenge-antigen enamathelayo.

Inqubo yokuhlola iqala ngokwengeza isampula ye-serum emithonjeni yokusabela embozwe ngaphambili nge-antigen, elandelwa ukufukamela. Uma i-ZP-Ab ikhona kusampula, izobophezela ngokukhethekile ku-antigen eboshiwe ye-zona pellucida emithonjeni, yakhe izakhi ezizinzile ze-antigen-antibody.

Okulandelayo, ama-enzyme conjugates anezelwa emithonjeni. Ngemva kwesinyathelo sesibili sokufukamela, lezi zihlanganisi ze-enzyme zibophezela kuma-antigen-antibody complexes akhona. Lapho kwethulwa isisombululo se-TMB substrate, ukusabela kombala kwenzeka ngaphansi kwesenzo se-enzyme eyinkimbinkimbi. Ekugcineni, isifundi se-microplate sisetshenziselwa ukukala ukumuncwa (A value), okuvumela ukunqunywa kwamaleveli e-ZP-Ab kusampula.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	EngaqondileIndlela
Isitifiketi	NI-MPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T /96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	12izinyanga