I-Anti-Trophoblast Cell Membrane (TA) Antibody ELISA Kit

Incazelo emfushane:

Lo mkhiqizo usetshenziselwa ukutholwa kwekhwalithi ye-in vitro yama-anti-trophoblast cell membrane amasosha omzimba ku-serum yomuntu. Amaseli e-Trophoblast ayizingxenye ezibalulekile ze-placenta, adlala indima ebalulekile ekufakweni kombungu kusenesikhathi, ukwakheka kwe-placenta, nokugcina ukubekezelelana okujwayelekile kokuzivikela komama nengane.

Ama-anti-trophoblast cell membrane amasosha omzimba angama-autoantibodies aqondise ama-antigen ebusweni bamaseli e-trophoblast. Lapho la masosha omzimba evela emzimbeni, angase ahlasele amangqamuzana e-trophoblast, alimaze ukwakheka kwawo nokusebenza kwawo, aphazamise ukufakwa okuvamile kwemibungu, futhi aphazamise ibhalansi yamasosha omzimba phakathi kukamama nombungu. Lokhu kungaholela ekuhlulekeni kokufakelwa, ukukhulelwa kusenesikhathi, noma okunye ukuphazamiseka kokuzala, kube yimbangela engaba yimbangela yokungazali kwe-autoimmune.

Ngokomtholampilo, lokhu kutholwa kusebenza njengethuluzi elisizayo lokuxilonga lokungazali kwe-autoimmune. Kuyasiza ukubona ukuthi ukulimala kwamasosha omzimba kumaseli e-trophoblast kuyabandakanyeka yini ku-pathogenesis yokungazali, ukunikeza ulwazi olubalulekile lwereferensi kubahlengikazi ukuze bacacise izimbangela zokungazali kanye nokuthuthukisa amasu okwelapha afanelekile.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le khithi ithola amasosha omzimba e-trophoblast cell membrane (TA-Ab) kumasampula e-serum yomuntu asekelwe endleleni engaqondile, nama-membrane eseli e-trophoblast ahlanzekile asetshenziswa njenge-antigen yokumboza.

Inqubo yokuhlola iqala ngokwengeza isampula ye-serum emithonjeni yokusabela embozwe ngaphambili nge-antigen, okulandelwa ukufukamela. Uma i-TA-Ab ikhona kusampula, izobophezela ngokukhethekile kuma-antigen olwelwesi lwamaseli e-trophoblast amboziwe emithonjeni, yakhe izinhlanganisela ezizinzile ze-antigen-antibody.

Ngemva kokukhipha izingxenye ezingaboshiwe ngokugeza ukuze kuqinisekiswe ukunemba kokutholwa, ama-enzyme conjugates ayengezwa emithonjeni. Ukufakwa kwesibili kwe-incubation kuvumela lezi zihlanganisi ze-enzyme ukuthi zibophezele kuma-antigen-antibody complexes akhona. Lapho kwethulwa isisombululo se-TMB substrate, i-enzyme eyinkimbinkimbi idala ukusabela nge-TMB, ikhiqize ushintsho olubonakalayo lombala. Ekugcineni, isifundi se-microplate sikala ukumuncwa (A value), okusetshenziselwa ukunquma izinga le-TA-Ab kusampula.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	EngaqondileIndlela
Isitifiketi	NI-MPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T /96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	12izinyanga