I-Anti-Ovarian (AO) Antibody ELISA Kit

Incazelo emfushane:

I-ovary iqukethe amaqanda, i-zona pellucida, amaseli e-granulosa, njll., ezigabeni zokukhula ezihlukene. Ingxenye ngayinye ingase ifake ama-anti-ovarian anti-ovarian (AoAb) ngenxa ye-antigen engavamile. Ukuchitheka kwe-ovarian antigen okubangelwa ukulimala kwesibeletho, ukutheleleka, noma ukuvuvukala kungase kubangele i-AoAb kubantu abanokukhubazeka komzimba. I-AoAb iphinde ilimaze i-ovary futhi ikhinyabeze ukusebenza kwesibeletho kanye ne-placenta, ibangele ukungazali nokuphuphuma kwesisu.

I-AoAb yatholwa okokuqala ezigulini ezinokwehluleka kwe-ovarian ngaphambi kwesikhathi (POF) kanye ne-amenorrhea yakuqala, exhunywe nokusabela kwe-autoimmune. I-AoAb ekuqaleni yehlisa ukuzala futhi ekugcineni iholele ekuhlulekeni kwe-ovarian. Iziguli ezingazali ezine-AoAb evumayo kodwa ingekho i-POF ingase ibhekane nezingozi ze-POF eziphakeme zesikhathi esizayo, ezidinga ukuhlolwa kwe-ovarian reserve.

I-AoAb positivity iphezulu ezigulini ezingazali kanye neziphuphunyelwa yizisu, okubonisa ubudlelwano obuseduze. Ucwaningo lukhombisa ukuthi i-AoAb idala ukungazali kunokuphuphunyelwa yisisu. Ucwaningo lwakamuva luthola i-AoAb ezigulini eziningi ze-PCOS, luphakamisa ukuthi ukuvuvukala kwe-ovarian okubangelwa amasosha omzimba kanye nama-cytokines angajwayelekile kungase kubangele i-PCOS kanye nokungazali, okudinga ukucwaninga okwengeziwe.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le khithi ithola amasosha omzimba alwa namaqanda (IgG) kumasampuli e-serum yomuntu ngokusekelwe endleleni engaqondile, ngama-antigen acwengiwe olwelwesi lwesibeletho asetshenziselwa ukumboza ama-microwells ngaphambili.

Inqubo yokuhlola iqala ngokungeza isampula ye-serum emithonjeni yokusabela e-antigen-precoated ukuze kufakwe i-incubation. Uma amasosha omzimba e-anti-ovarian ekhona kusampula, azobophezela ngokukhethekile kuma-antigens olwelwesi lwesibeletho oluvalwe ngaphambili kuma-microwells, enze ama-antigen-antibody complexes azinzile. Izingxenye ezingaboshiwe zibe sezikhishwa ukuze kuqinisekiswe ukunemba kokutholwa.

Okulandelayo, i-horseradish peroxidase (HRP) -amasosha omzimba egundane abhalwe ukuthi anti-human IgG afakwa emithonjeni. Ngemva kokufukamela okwesibili, lawa masosha omzimba abhalwe nge-enzyme abopha ngokuqondile amasosha omzimba kuma-antigen-antibody complexes akhona, enza "ilebula le-antigen-antibody-enzyme" eliphelele.

Ekugcineni, isisombululo se-TMB substrate siyengezwa. I-HRP endaweni eyinkimbinkimbi idala ukusabela kwamakhemikhali nge-TMB, ikhiqize ukuguqulwa kombala okubonakalayo. Ukumunca (Inani elingu-A) lesixazululo sokusabela kukalwa kusetshenziswa isifundi se-microplate, futhi ukuba khona noma ukungabikho kwamasosha omzimba alwa namaqanda kusampula kunqunywa ngokusekelwe kumphumela wokumunca.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	EngaqondileIndlela
Isitifiketi	NI-MPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T /96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	12izinyanga