I-Anti-Islet Cell (ICA) Antibody ELISA Kit

Incazelo emfushane:

Le kit yakhelwe ukuthola ikhwalithi ye-in vitro amazinga e-anti-islet cell antibody (ICA) ku-serum yomuntu. Ngokomtholampilo, isetshenziswa kakhulu njengethuluzi elisizayo lokuxilonga le-Type 1 Diabetes Mellitus (T1DM).

Amasosha omzimba ama-Islet cell angama-autoantibodies aqondise ama-antigen angaphezulu noma ngaphakathi kwamaseli e-pancreatic islet, ikakhulukazi ama-β cell. Ukuba khona kwabo kuhlobene eduze nokulimala kwe-autoimmune kumaseli e-islet, okuyisici esibalulekile se-T1DM se-pathological. Ezigabeni zokuqala ze-T1DM, ngisho nangaphambi kokuba kuvele izimpawu zomtholampilo ezisobala njenge-hyperglycemia, i-ICA ingatholakala ku-serum, okuyenza ibe uphawu olubalulekile lokuzivikela komzimba ekuqaleni kwalesi sifo.

Kubantu abanomlando womndeni wesifo sikashukela noma labo ababonisa izimpawu zangaphambi kwesifo sikashukela, ukuthola amazinga e-ICA kusiza ukuhlola ubungozi bokuthuthukisa i-T1DM. Ukwengeza, ezigulini ezinezimbangela ezingacacile ze-hyperglycemia, izinsiza zokuhlola i-ICA ekuhlukaniseni i-T1DM kwezinye izinhlobo zesifo sikashukela, ngaleyo ndlela iqondise ukwakhiwa kwezinhlelo zokwelashwa ezifanele. Ngokuqapha izinguquko emazingeni e-ICA, inganikeza nereferensi yokuhlola ukuqhubeka kokulimala kwe-islet cell kanye nempumelelo yezinyathelo zokungenelela.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le khithi ithola amasosha omzimba e-islet cell (ICA) kumasampuli e-serum yomuntu ngokusekelwe endleleni engaqondile, nama-antigens ahlanziwe we-islet cell asetshenziswa njenge-antigen enamathelayo.

Inqubo yokuhlola iqala ngokwengeza isampula ye-serum emithonjeni yokusabela embozwe ngaphambili nge-antigen, elandelwa ukufukamela. Uma i-ICA ikhona kusampula, izobophezela ngokukhethekile kuma-antigen eseli e-islet amboziwe emithonjeni, yakhe ama-antigen-antibody complexes azinzile. Izingxenye ezingaboshiwe zibe sezikhishwa ngokuwashwa ukuze kuqinisekiswe ukunemba kokusabela okulandelayo.

Okulandelayo, ama-enzyme conjugates anezelwa emithonjeni. Ngemva kwesinyathelo sesibili sokufukamela, lezi zihlanganisi ze-enzyme zibophezela kuma-antigen-antibody complexes akhona. Lapho kwethulwa isisombululo se-TMB substrate, i-enzyme eyinkimbinkimbi idala ukusabela nge-TMB, okuholela ekushintsheni kombala okubonakalayo. Okokugcina, isifundi se-microplate sisetshenziselwa ukukala ukumunca (Ivelu engu-A), okuvumela ukunqunywa kwamazinga e-ICA kusampula ngokusekelwe kubukhulu bokusabela kombala.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	EngaqondileIndlela
Isitifiketi	NI-MPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T /96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	12izinyanga