I-Anti-Endometrial (EM) Antibody ELISA Kit

Incazelo emfushane:

Le kit isetshenziselwa ukuthola ikhwalithi ye-in vitro yamasosha omzimba alwa ne-endometrial (EmAb) ku-serum yomuntu.

I-EmAb iyi-autoantibody eqondise i-endometrium, edala izimpendulo zamasosha omzimba. Kuyi-antibody engumaka ye-endometriosis futhi ehlotshaniswa nokuphuphuma kwesisu kwabesifazane kanye nokungazali. Imibiko ikhombisa u-37% -50% weziguli ezinokungazali, eziphuphunyelwa yisisu noma i-endometriosis zine-EmAb-positive; izinga lifinyelela ku-24% -61% kwabesifazane ngemva kokuhushula izisu okuzenzela.

I-EmAb ibophezela kuma-antigen e-endometrial, i-endometrium elimazayo ngokwenza kusebenze okuhambisanayo nokuqashwa kwamangqamuzana omzimba omzimba, ikhinyabeze ukufakwa kombungu futhi kubangele ukuphuphuma kwesisu. Ivamise ukuhlangana ne-endometriosis, enezinga lokutholwa lika-70% -80% ezigulini ezinjalo. Le kit isiza ukuxilonga i-endometriosis, ibheke imiphumela yokwelashwa, futhi ithuthukise imiphumela yokungazali okuhlobene.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le khithi ithola amasosha omzimba alwa ne-endometrial (IgG) kumasampuli e-serum yomuntu ngokususelwe endleleni engaqondile, nama-antigen acwengiwe olwelwesi lwe-endometrial asetshenziselwa ukumboza ama-microwells ngaphambili.

Inqubo yokuhlola iqala ngokwengeza isampula ye-serum emithonjeni yokusabela e-antigen-precoated ukuze kufakwe i-incubation. Uma amasosha omzimba alwa ne-endometrial ekhona kusampula, azobophezela ngokuqondile kuma-antigen e-endometrial ambozwe ngaphambili kuma-microwells, enze inhlanganisela yama-antigen-antibody ezinzile. Ngemva kokukhipha izingxenye ezingaboshiwe ngokuwasha ukuze kugwenywe ukuphazamiseka, amasosha omzimba e-horseradish peroxidase anelebula legundane elilwa ne-IgG yomuntu.

Ukulandela okunye ukufukamela, lawa amasosha omzimba abhalwe ngama-enzyme abophezela kuma-antigen-antibody complexes akhona. Lapho i-substrate ye-TMB yengezwa, ukusabela kombala kwenzeka ngaphansi kwe-catalysis ye-enzyme. Ekugcineni, isifundi se-microplate sikala ukumunca (A value), okusetshenziselwa ukunquma ukuba khona kwamasosha omzimba alwa ne-endometrial (IgG) kusampula.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	EngaqondileIndlela
Isitifiketi	NI-MPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T /96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	12izinyanga