I-Anti-Cyclic Citrullinated Peptide (CCP) Antibody ELISA Kit

Incazelo emfushane:

Lo mkhiqizo uhloselwe ukutholwa kwekhwalithi ye-in vitro yamazinga e-anti-cyclic citrullinated peptide antibody ku-serum yomuntu. Ngokomtholampilo, iyasebenza njengethuluzi lokuxilonga elisizayo le-rheumatoid arthritis (RA).

Amasosha omzimba e-Anti-cyclic citrullinated peptide ama-autoantibodies aqondise ama-peptide e-citrullinated cyclic, uhlobo lwe-protein antigen eguquliwe. Ukuba khona kwabo kuhlotshaniswa eduze nesifo samathambo, isifo esingamahlalakhona sokuzivikela komzimba esibonakala ngokuvuvukala kwamalunga nokulimala. Uma kuqhathaniswa nezinye izimpawu ze-rheumatoid, lawa masosha omzimba abonisa ukucaciswa okuphezulu kwe-RA, ikakhulukazi ezigabeni zokuqala zesifo lapho izimpawu zomtholampilo zingakabonakali.

Ezigulini ezine-RA esolwayo, ukuthola amazinga e-anti-cyclic citrullinated antibody e-peptide kungasiza ukuqinisekisa ukuxilongwa kusenesikhathi, okubalulekile ekungeneleleni okufika ngesikhathi kanye nokuvimbela ukulimala okuhlangene okungalungiseki. Kuyasiza futhi ekuhlukaniseni i-RA kwezinye izinhlobo zesifo samathambo ezinezimpawu ezifanayo, ngaleyo ndlela iqondise odokotela ukuba bathuthukise izinhlelo zokwelapha ezihlosiwe kanye nokwenza ngcono ukuphathwa okuphelele kwesifo.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

Le khithi ithola amasosha omzimba e-anti-cyclic citrullinated peptide (amasosha omzimba e-CCP) kumasampuli e-serum yabantu ngokusekelwe endleleni engaqondile, nama-antigen e-peptide e-cyclic citrullinated ahlanzekile asetshenziswa njenge-antigen embozayo.

Inqubo yokuhlola iqala ngokwengeza isampula ye-serum emithonjeni yokusabela eye yambozwa ngaphambili ngama-antigen acwengiwe ashiwo ngenhla, okulandelwa isikhathi sokufukamela. Phakathi nalokhu kufakwa, uma amasosha omzimba e-CCP ekhona kusampula, azobona ngokuqondile futhi abophezele kuma-antigen e-peptide e-citrullinated embozwe kuma-microwells, enze ama-antigen-antibody complexes azinzile. Ukuqinisekisa ukunemba kwezinyathelo ezilandelayo, izingxenye ezingaboshiwe emithonjeni yokusabela zisuswa ngenqubo yokugeza, okusiza ukuqeda ukuphazamiseka okungenzeka kwezinye izinto ku-serum.

Okulandelayo, ama-enzyme conjugates anezelwa emithonjeni yokusabela. Ngemuva kokufakwa kwesibili, lezi zihlanganisi ze-enzyme zizonamathisela ngokuqondile kuma-antigen-antibody complexes akhona, enze inkimbinkimbi enkulu yamasosha omzimba ehlanganisa i-antigen, i-antibody, ne-enzyme conjugate. Lapho isixazululo se-TMB substrate singeniswa ohlelweni, i-enzyme eku-conjugate ivuselela ukusabela kwamakhemikhali nge-substrate ye-TMB, okuholela ekushintsheni kombala okubonakalayo. Ukushuba kwalokhu kusabela kombala kuhlobene ngokuqondile nenani lamasosha omzimba e-CCP akhona kusampula yokuqala ye-serum. Ekugcineni, isifundi se-microplate sisetshenziswa ukukala ukumuncwa (A value) kwengxube yokusabela. Ngokuhlaziya leli nani lokumunca, izinga lamasosha omzimba e-CCP kusampula linganqunywa ngokunembile, linikeze isisekelo esithembekile sokuhlolwa okufanelekile komtholampilo kanye nokuxilongwa.

Izici Zomkhiqizo

Ukuzwela okuphezulu, ukucaciswa nokuzinza

Ukucaciswa Komkhiqizo

Isimiso	Ukuhlolwa kwe-Enzyme okuhlotshaniswa ne-immunosorbent
Uhlobo	EngaqondileIndlela
Isitifiketi	NI-MPA
Isifanekiso	I-serum yomuntu / i-plasma
Ukucaciswa	48T /96T
Izinga lokushisa lesitoreji	2-8℃
Impilo yeshelufu	12izinyanga