Ukuxilongwa kwe-HFRS - I-Hemorrhagic Fever ene-Renal Syndrome

Ingemuva

I-Hantaan virus (HV) iyigciwane lesandulela ngculazi elibangela i-Hemorrhagic Fever ene-Renal Syndrome (HFRS). I-HFRS isifo esithathelwanayo esisatshalaliswa emhlabeni wonke esibonakala ngomkhuhlane, ukopha, kanye nokukhubazeka kwezinso. Lesi sifo siqala kakhulu, sikhula ngokushesha, kanye nezinga eliphezulu lokufa, okubangela usongo olukhulu empilweni yomphakathi. Amagundane (afana ne-Apodemus agrarius kanye ne-Rattus norvegicus) ama-reservoir amakhulu nama-vectors e-HV. Ukudluliselwa kubantu kwenzeka kakhulukazi ngendle ene-aerosolized (umchamo, indle, amathe), ukuthintana ngokuqondile, noma ukulunywa yi-vector. I-HFRS ingenzeka unyaka wonke, futhi abantu abaningi bayathinteka. Ngokwezibalo ze-WHO, amazwe angama-32 emhlabeni wonke abike ukuqubuka kwe-HV, ikakhulukazi eMpumalanga Asia, eYurophu nase-Balkan.

Izimpawu Ze-Antibody Post Ukutheleleka kwe-HV

Ukulandela ukutheleleka nge-HV, amasosha omzimba womuntu akhiqiza amasosha omzimba athile, ikakhulukazi i-HV-IgM ne-HV-IgG.

● Amasosha omzimba e-HV-IgM: Asebenza njengophawu lwe-serological lokutheleleka kwangaphambi kwesikhathi, ngokuvamile avela phakathi nezinsuku zokuqala kwezimpawu, futhi abalulekile ekuxilongweni kwesigaba esibucayi.

● Amasosha omzimba e-HV-IgG: Avela kamuva futhi angaphikelela ukuphila kwakhe konke, okubonisa ukutheleleka kwangaphambilini noma ukululama. Ukwenyuka okuphindwe kane noma okukhulu kwe-HV-IgG antibody titer phakathi kwamasampula e-acute kanye ne-convalescent serum nakho kuwukuxilongwa kwegciwane eliyingozi.

Izindlela Zokuxilonga Ezijwayelekile ze-HV

Izindlela zamanje zaselabhorethri zokutholwa kwe-HV zifaka ukuhlukaniswa kwegciwane, i-PCR, i-serological ELISA, kanye nama-colloidal gold immunoassays.

● Isiko legciwane kanye ne-PCR kunikeza ukucaciswa okuphezulu kodwa kudla isikhathi, kufuna ngobuchwepheshe, futhi kudinga izindawo zaselabhorethri ezithuthukisiwe, ezinciphisa ukusetshenziswa kwazo okusabalele.

● I-Micro-immunofluorescence (MIF) inikeza ukunemba okuhle kodwa idinga isibonakhulu se-fluorescence kanye nokutolika kochwepheshe, okukhawulela ukusetshenziswa okujwayelekile.

● I-ELISA kanye nokuhlolwa kwegolide kwe-colloidal kwamukelwa kabanzi kuzilungiselelo zomtholampilo ngenxa yobulula bazo, isivinini, ukuzwela okuphezulu nokucaciswa kwayo, kanye nokuqoqwa kalula kwesampula (i-serum/plasma).

Ukusebenza Komkhiqizo

Izici ze-Beier Bio's HV-IgM/IgG (ELISA) zokuhlola

● Uhlobo Lwesampula: I-Serum, i-plasma

● Ukwehliswa Kwesampula: Kokubili izivivinyo ze-IgM ne-IgG zisebenzisa isampuli yomthombo yoqobo ene-dilution engu-1:11 (isampula ye-100µl diluent + 10µl isampula), eqondisa ukuhamba komsebenzi futhi yehlise umthwalo womsebenzisi.

● I-Reagent Ilungele Ukusetshenziswa: Wonke ama-reagents alungile ngaphandle kwebhafa yokugeza (20× kugxilile). Ifakwe ikhodi yombala ukuze ibonakale kalula

● Inqubo yokufukamela: 30 min / 30 min / 15 min; kuyazenzekela ngokugcwele

● Ubude be-Wavelength bokutholwa: 450 nm nereferensi engu-630 nm

● Imichilo Ehlanganisiwe: 96 noma 48 imithombo ephukayo, ngayinye enekhodi yomkhiqizo ephrintiwe ukuze kulandeleke futhi kube lula.

Izici Zokuhlola ze-Beier Bio's HV-IgM/IgG (Colloidal Gold)

● Uhlobo Lwesampula: I-Serum

● Isikhathi Sokuthola: Imiphumela phakathi nemizuzu engu-15; akukho mishini eyengeziwe edingekayo; ilungele ukuhlolwa ngokushesha ezigulini ezilaliswayo, eziphuthumayo, nezilungiselelo zesiguli ezihlakazekile

● Inqubo: Engeza isampula engu-10µl kusampula yekhadi lokuhlola kahle usebenzisa i-dropper; humusha imiphumela phakathi kwemizuzu eyi-15–20

Ukusebenza Komtholampilo kwe-HV-IgM (ELISA), HV-IgG (ELISA), kanye ne-HV-IgM/IgG (Colloidal Gold)